Chip-Capillary Hybrid Device for Automated Transfer of Sample Preseparated by Capillary Isoelectric Focusing to Parallel Capillary Gel Electrophoresis for Two-Dimensional Protein Separation
نویسندگان
چکیده
منابع مشابه
Two-dimensional capillary electrophoresis: capillary isoelectric focusing and capillary zone electrophoresis with laser-induced fluorescence detection.
CIEF and CZE are coupled with LIF detection to create an ultrasensitive 2-D separation method for proteins. In this method, two capillaries are joined through a buffer-filled interface. Separate power supplies control the potential at the injection end of the first capillary and at the interface; the detector is held at ground potential. Proteins are labeled with the fluorogenic reagent Chromeo...
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An improvement has been made to couple isoelectric focusing (IEF) sample injection and capillary zone electrophoresis in an untreated fused silica capillary. Electroosmotic flow is efficiently prevented by simply using a rubber block at the outlet end of the capillary during IEF sample injection. The experimental conditions that affect the concentration effect are discussed. A concentration enh...
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Two−dimensional polyacryiamide gel electrophoresis (2−D PAGE) has been widely used for protein separation because of its high resolution; more than one thousand proteins or polypeptides can be separated according to the differences of their isoelectric point (pI) and molecular mass. However, 2−D PAGE involves many steps of manual handling, sample injection, setting gel molds in an electrophores...
متن کاملOn-line combination of capillary isoelectric focusing and capillary non-gel sieving electrophoresis using a hollow-fiber membrane interface: a novel two-dimensional separation system for proteins.
A novel two-dimensional (2D) separation system for proteins was reported. In the system, a piece of dialysis hollow-fiber membrane was employed as the interface for on-line combination of capillary isoelectric focusing (CIEF) and capillary non-gel sieving electrophoresis (CNGSE). The system is similar equivalent to two-dimensional polyacrylamide gel electrophoresis (2D PAGE), by transferring th...
متن کاملCapillary Isoelectric Focusing
Beale SC (1998) Capillary electrophoresis. Analytical Chemistry 70: 279. (This journal prints every two years a review on capillary electrophoresis. The article is written by an expert in the Reld and covers more or less all developments in CE including CGE of the preceding years.) Grossmann PD and Colburn JC (eds) (1996) Capillary Electrophoresis: Theory and Practice. San Diego: Academic Press...
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ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2012
ISSN: 0003-2700,1520-6882
DOI: 10.1021/ac3017168